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| Prasad,Polu Giri; Jasmine,Mohammad Shaik; kanta,Kota Neela Mani; Deepthi,Karumanchi; Allam,Uday Sankar. |
| ABSTRACT Purpose and objectives Detection of drug resistance plays a crucial role in tuberculosis (TB) treatment and prevention of Mycobacterium tuberculosis (MTB) transmission. The aim of this study was to determine the levels and patterns of resistance of MTB isolates to two key anti-TB drugs (rifampicin, RIF and isoniazid, INH) and the type of mutations in drug resistance genes (rpoB, katG and inhA) of the isolates at the southern coastal region of Andhra Pradesh, India, using commercially available GenoType MTBDRplus assay under the Revised National TB Control Program. Methods GenoType MTBDRplus assay was performed on 2859 sputum smear-positive samples and the mutations in the genes responsible for resistance (rpoB, katG and inhA) were analyzed.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Multidrug resistance; Mycobacterium tuberculosis; MTBDRplus assay; Mutations; Molecular detection. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500281 |
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| Weiblen,Carla; Azevedo,Maria Isabel de; Ianiski,Lara Baccarin; Stibbe,Paula Cristina; Pereira,Daniela Isabel Brayer; Zanette,Régis Adriel; Sangioni,Luís Antônio; Rivero,Rodolfo; Santurio,Janio Morais; Botton,Sônia de Avila. |
| ABSTRACT: We aimed to genotype the South American clinical isolates of Pythium insidiosum using the single nucleotide polymorphisms (SNP) of the ribosomal DNA sequences (rDNA). Previously, an SNP-based multiplex-PCR was able to distinguish three different clades of P. insidiosum isolates. Thus, we used this assay to evaluate South American clinical isolates of P. insidiosum (n=32), standard strains from Costa Rica (n=4), Thailand (n=3), Japan (n=1), and India (n=1), a standard strain of Pythium aphanidermatum, and Brazilian environmental isolates of Pythium torulosum, Pythium rhizo-oryzae and Pythium pachycaule voucher (n=3). It was possible to allocate each American P. insidiosum isolate to clade I, the isolates of India, Japan, and Thailand to clade II,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pythium insidiosum; Pythiosis; Molecular detection; Genotype; Single nucleotide polimorphisms. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100450 |
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| CARDOSO,Gabrielle Virgínia Ferreira; OLIVEIRA,Andrey Carlos do Sacramento de; SILVA,Andréia Silva da; SILVA,Marcos Clécio de Lemos; LIMA,Joelson Sousa; ROOS,Talita Bandeira; MORAES,Carina Martins de. |
| ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This study aimed to optimize polymerase chain reaction (PCR)-based detection of T. cruzi DNA in açai pulp. Several dilutions of T. cruzi DTU TcI trypomastigote forms were cultured in liver infusion tryptose (LIT) medium. Trypanosoma cruzi DNA was extracted from the cells and subjected to PCR. Subsequently, culture dilutions were added to açai pulp to evaluate the detection threshold of the optimized PCR assay. We demonstrate that our assay can detect T. cruzi DNA in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection method; Foodborne disease; Food pathogen; Molecular detection; Chagas disease. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079 |
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